Virtual Terminator nucleotides for next generation DNA sequencing

We synthesized reversible terminators with tethered inhibitors for next generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides and were used to sequence canine bacterial artificial chromosomes in a single-molecule system...

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Main Authors: Bowers, Jayson, Mitchell, Judith, Beer, Eric, Buzby, Philip R., Causey, Marie, Efcavitch, J. William, Jarosz, Mirna, Krzymanska-Olejnik, Edyta, Kung, Li, Lipson, Doron, Lowman, Geoffrey M., Marappan, Subramanian, McInerney, Peter, Platt, Adam, Roy, Atanu, Siddiqi, Suhaib M., Steinmann, Kathleen, Thompson, John F.
Format: Artigo
Jezik:Inglês
Izdano: 2009
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2719685/
https://ncbi.nlm.nih.gov/pubmed/19620973
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1354
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