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Virtual Terminator nucleotides for next generation DNA sequencing

We synthesized reversible terminators with tethered inhibitors for next generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides and were used to sequence canine bacterial artificial chromosomes in a single-molecule system...

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Bibliografiske detaljer
Main Authors: Bowers, Jayson, Mitchell, Judith, Beer, Eric, Buzby, Philip R., Causey, Marie, Efcavitch, J. William, Jarosz, Mirna, Krzymanska-Olejnik, Edyta, Kung, Li, Lipson, Doron, Lowman, Geoffrey M., Marappan, Subramanian, McInerney, Peter, Platt, Adam, Roy, Atanu, Siddiqi, Suhaib M., Steinmann, Kathleen, Thompson, John F.
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2719685/
https://ncbi.nlm.nih.gov/pubmed/19620973
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1354
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