Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.
We report the design, synthesis and evaluation of a non-nucleosidic photocleavable biotin phosphoramidite (PCB-phosphoramidite) which provides a simple method for purification and phosphorylation of oligonucleotides. This reagent introduces a photocleavable biotin label (PCB) on the 5'-terminal phos...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1996
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145639/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8628663/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.2.361 |
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