Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.

We report the design, synthesis and evaluation of a non-nucleosidic photocleavable biotin phosphoramidite (PCB-phosphoramidite) which provides a simple method for purification and phosphorylation of oligonucleotides. This reagent introduces a photocleavable biotin label (PCB) on the 5'-terminal phos...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Olejnik, J, Krzymańska-Olejnik, E, Rothschild, K J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1996
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145639/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8628663/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.2.361
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