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Fluoride-cleavable biotinylation phosphoramidite for 5′-end-labeling and affinity purification of synthetic oligonucleotides

A fluoride-cleavable phosphoramidite for biotinylation was designed, synthesized and coupled efficiently to the 5′-end of DNA on an automatic synthesizer. The diisopropylsilyl acetal functionality was used to link the biotin moiety through a tertiary hydroxide group to the 5′-end of DNA. This linkag...

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Detalhes bibliográficos
Main Authors: Fang, Shiyue, Bergstrom, Donald E.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC140496/
https://ncbi.nlm.nih.gov/pubmed/12527780
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