Fluoride-cleavable biotinylation phosphoramidite for 5′-end-labeling and affinity purification of synthetic oligonucleotides
A fluoride-cleavable phosphoramidite for biotinylation was designed, synthesized and coupled efficiently to the 5′-end of DNA on an automatic synthesizer. The diisopropylsilyl acetal functionality was used to link the biotin moiety through a tertiary hydroxide group to the 5′-end of DNA. This linkag...
Na minha lista:
| Publicado no: | Nucleic Acids Res |
|---|---|
| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2003
|
| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC140496/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12527780/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkg130 |
| Tags: |
Sem tags, seja o primeiro a adicionar uma tag!
|