Fluoride-cleavable biotinylation phosphoramidite for 5′-end-labeling and affinity purification of synthetic oligonucleotides

A fluoride-cleavable phosphoramidite for biotinylation was designed, synthesized and coupled efficiently to the 5′-end of DNA on an automatic synthesizer. The diisopropylsilyl acetal functionality was used to link the biotin moiety through a tertiary hydroxide group to the 5′-end of DNA. This linkag...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Fang, Shiyue, Bergstrom, Donald E.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2003
Schlagworte:	Articles
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC140496/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12527780/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkg130
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