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Rapid generation of random mutant libraries
A simple and efficient method utilizing in vivo recombination to create recombinant libraries incorporating the products of PCR amplification is described. This will be especially useful for generating large pools of randomly mutagenized clones after error-prone PCR mutagenesis. Here we investigate...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Landes Bioscience
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3037583/ https://ncbi.nlm.nih.gov/pubmed/21326833 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/bbug.1.5.12942 |
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