Next-generation genome sequencing can be used to rapidly characterise sequences flanking T-DNA insertions in random insertional mutants of Leptosphaeria maculans

BACKGROUND: Banks of mutants with random insertions of T-DNA from Agrobacterium tumefaciens are often used in forward genetics approaches to identify phenotypes of interest. Upon identification of mutants of interest, the flanking sequences of the inserted T-DNA must be identified so that the mutate...

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Detalhes bibliográficos
Publicado no:Fungal Biol Biotechnol
Main Authors: Chambers, Kylie, Lowe, Rohan GT, Howlett, Barbara J, Zander, Manuel, Batley, Jacqueline, Van de Wouw, Angela P, Elliott, Candace E
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Short Report
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5611616/
https://ncbi.nlm.nih.gov/pubmed/28955452
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40694-014-0010-y
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