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Toxoflavin Lyase Requires a Novel 1-His-2-Carboxylate Facial Triad,

High resolution crystal structures are reported for apo, holo, and substrate-bound forms of a toxoflavin-degrading metalloenzyme (TflA). In addition, the degradation reaction is shown to be dependent on oxygen, Mn(II), and dithiothreitol in vitro. Despite its low sequence identity with proteins of k...

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Détails bibliographiques
Auteurs principaux: Fenwick, Michael K., Philmus, Benjamin, Begley, Tadhg P., Ealick, Steven E.
Format: Artigo
Langue:Inglês
Publié: 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3035768/
https://ncbi.nlm.nih.gov/pubmed/21166463
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi101741v
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