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Improved Precursor Directed Biosynthesis in E. coli via Directed Evolution

Erythromycin and related macrolide antibiotics are widely used polyketide natural products. We have evolved an engineered biosynthetic pathway in Escherichia coli that yields erythromycin analogs from simple synthetic precursors. Multiple rounds of mutagenesis and screening led to the identification...

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Bibliografski detalji
Glavni autori: Lee, Ho Young, Harvey, Colin J.B., Cane, David E., Khosla, Chaitan
Format: Artigo
Jezik:Inglês
Izdano: 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3030684/
https://ncbi.nlm.nih.gov/pubmed/21081955
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ja.2010.129
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