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Effect of PCR extension temperature on high-throughput sequencing
The DNA amplification process can be a source of bias and artifacts, especially when amplifying genomic areas with extreme AT or GC content. The human malaria parasite Plasmodium falciparum has an AT-rich genome, and some of its highly AT-rich regions have been shown to be refractory to polymerase c...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3026866/ https://ncbi.nlm.nih.gov/pubmed/21112355 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molbiopara.2010.11.013 |
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