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Digital PCR provides sensitive and absolute calibration for high throughput sequencing
BACKGROUND: Next-generation DNA sequencing on the 454, Solexa, and SOLiD platforms requires absolute calibration of the number of molecules to be sequenced. This requirement has two unfavorable consequences. First, large amounts of sample-typically micrograms-are needed for library preparation, ther...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2667538/ https://ncbi.nlm.nih.gov/pubmed/19298667 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-10-116 |
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