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Sources of PCR-induced distortions in high-throughput sequencing data sets

PCR permits the exponential and sequence-specific amplification of DNA, even from minute starting quantities. PCR is a fundamental step in preparing DNA samples for high-throughput sequencing. However, there are errors associated with PCR-mediated amplification. Here we examine the effects of four i...

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書目詳細資料
發表在:Nucleic Acids Res
Main Authors: Kebschull, Justus M., Zador, Anthony M.
格式: Artigo
語言:Inglês
出版: Oxford University Press 2015
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666380/
https://ncbi.nlm.nih.gov/pubmed/26187991
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv717
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