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Structural and aggregation behavior of the human γD-crystallin mutant E107A, associated with congenital nuclear cataract

PURPOSE: To analyze the conformational features and aggregation properties of the mutant protein E107A human γD-crystallin (HGDC), associated with congenital nuclear cataract. METHODS: cDNAs of wild type and E107A mutant were cloned and expressed in BL21 (DE3) pLysS cells and the proteins isolated a...

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Bibliografiske detaljer
Main Authors: Vendra, Venkata Pulla Rao, Balasubramanian, Dorairajan
Format: Artigo
Sprog:Inglês
Udgivet: Molecular Vision 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3008718/
https://ncbi.nlm.nih.gov/pubmed/21197114
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