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Targeted insertion of cysteine by decoding UGA codons with mammalian selenocysteine machinery
Cysteine (Cys) is inserted into proteins in response to UGC and UGU codons. Herein, we show that supplementation of mammalian cells with thiophosphate led to targeted insertion of Cys at the UGA codon of thioredoxin reductase 1 (TR1). This Cys was synthesized by selenocysteine (Sec) synthase on tRNA...
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| Główni autorzy: | , , , , , , , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
National Academy of Sciences
2010
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3003055/ https://ncbi.nlm.nih.gov/pubmed/21115847 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1009947107 |
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