Targeted insertion of cysteine by decoding UGA codons with mammalian selenocysteine machinery

Cysteine (Cys) is inserted into proteins in response to UGC and UGU codons. Herein, we show that supplementation of mammalian cells with thiophosphate led to targeted insertion of Cys at the UGA codon of thioredoxin reductase 1 (TR1). This Cys was synthesized by selenocysteine (Sec) synthase on tRNA...

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Autores principales: Xu, Xue-Ming, Turanov, Anton A., Carlson, Bradley A., Yoo, Min-Hyuk, Everley, Robert A., Nandakumar, Renu, Sorokina, Irina, Gygi, Steven P., Gladyshev, Vadim N., Hatfield, Dolph L.
Formato: Artigo
Lenguaje:Inglês
Publicado: National Academy of Sciences 2010
Materias:
Biological Sciences
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3003055/
https://ncbi.nlm.nih.gov/pubmed/21115847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1009947107
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