Targeted insertion of cysteine by decoding UGA codons with mammalian selenocysteine machinery

Cysteine (Cys) is inserted into proteins in response to UGC and UGU codons. Herein, we show that supplementation of mammalian cells with thiophosphate led to targeted insertion of Cys at the UGA codon of thioredoxin reductase 1 (TR1). This Cys was synthesized by selenocysteine (Sec) synthase on tRNA...

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Bibliografiska uppgifter
Huvudupphovsmän: Xu, Xue-Ming, Turanov, Anton A., Carlson, Bradley A., Yoo, Min-Hyuk, Everley, Robert A., Nandakumar, Renu, Sorokina, Irina, Gygi, Steven P., Gladyshev, Vadim N., Hatfield, Dolph L.
Materialtyp: Artigo
Språk:Inglês
Publicerad: National Academy of Sciences 2010
Ämnen:
Biological Sciences
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3003055/
https://ncbi.nlm.nih.gov/pubmed/21115847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1009947107
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