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Selective Incorporation of Nitrile-Based Infrared Probes into Proteins via Cysteine Alkylation
The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins vi...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2010
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2999665/ https://ncbi.nlm.nih.gov/pubmed/21077670 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi101711a |
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