Purification, crystallization and preliminary crystallographic analysis of a multiple cofactor-dependent DNA ligase from Sulfophobococcus zilligii

A recombinant DNA ligase from Sulfophobococcus zilligii that shows multiple cofactor specificity (ATP, ADP and GTP) was expressed in Escherichia coli and purified under reducing conditions. Crystals were obtained by the microbatch crystallization method at 295 K in a drop containing 1 µl protein sol...

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Hauptverfasser: Supangat, Supangat, An, Young Jun, Sun, Younguk, Kwon, Suk-Tae, Cha, Sun-Shin
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2010
Schlagworte:
Crystallization Communications
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2998359/
https://ncbi.nlm.nih.gov/pubmed/21139200
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110034135
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