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Purification, crystallization and preliminary crystallographic analysis of biotin protein ligase from Staphylococcus aureus

Biotin protein ligase from Staphylococcus aureus catalyses the biotinylation of acetyl-CoA carboxylase and pyruvate carboxylase. Recombinant biotin protein ligase from S. aureus has been cloned, expressed and purified. Crystals were grown using the hanging-drop vapour-diffusion method using PEG 8000...

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Detalhes bibliográficos
Main Authors: Pendini, Nicole R., Polyak, Steve W., Booker, Grant W., Wallace, John C., Wilce, Matthew C. J.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2496860/
https://ncbi.nlm.nih.gov/pubmed/18540065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108012244
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