Purification, crystallization and preliminary crystallographic analysis of the biotin–protein ligase from Pyrococcus horikoshii OT3

Biotin–protein ligase is an enzyme that catalyzes the ATP-dependent biotinylation of a specific lysine residue in acetyl-CoA carboxylase. The biotin–protein ligase from Pyrococcus horikoshii OT3 has been cloned, overexpressed and purified. Crystallization was performed by the microbatch method or th...

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Bibliografiska uppgifter
Huvudupphovsmän: Bagautdinov, Bagautdin, Kuroishi, Chizu, Sugahara, Mitsuaki, Kunishima, Naoki
Materialtyp: Artigo
Språk:Inglês
Publicerad: International Union of Crystallography 2005
Ämnen:
Crystallization Communications
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952243/
https://ncbi.nlm.nih.gov/pubmed/16510991
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309104034360
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