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Purification, crystallization and preliminary crystallographic analysis of the vacuole-type ATPase subunit E from Pyrococcus horikoshii OT3

The vacuole-type ATPases in eukaryotic cells translocate protons across various biological membranes including the vacuolar membrane by consuming ATP molecules. The E subunit of the multisubunit complex V-ATPase from Pyrococcus horikoshii OT3, which has a molecular weight of 22.88 kDa, has been clon...

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Détails bibliographiques
Auteurs principaux: Lokanath, Neratur K., Ukita, Yoko, Sugahara, Mitsuaki, Kunishima, Naoki
Format: Artigo
Langue:Inglês
Publié: International Union of Crystallography 2004
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952376/
https://ncbi.nlm.nih.gov/pubmed/16508090
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309104026430
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