High-efficiency siRNA-based gene knockdown in human embryonic stem cells

Loss-of-function studies in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) via nonviral approaches have been largely unsuccessful. Here we report a simple and cost-effective method for high-efficiency delivery of plasmids and siRNAs into hESCs and iPSCs. Using this met...

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Bibliographische Detailangaben
Hauptverfasser: Ma, Yinghong, Jin, Jianyu, Dong, Chunsheng, Cheng, Ee-Chun, Lin, Haifan, Huang, Yingqun, Qiu, Caihong
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2010
Schlagworte:
Method
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2995416/
https://ncbi.nlm.nih.gov/pubmed/20978109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2350710
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