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High-efficiency siRNA-based gene knockdown in human embryonic stem cells
Loss-of-function studies in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) via nonviral approaches have been largely unsuccessful. Here we report a simple and cost-effective method for high-efficiency delivery of plasmids and siRNAs into hESCs and iPSCs. Using this met...
Gorde:
| Egile Nagusiak: | , , , , , , |
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| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
Cold Spring Harbor Laboratory Press
2010
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2995416/ https://ncbi.nlm.nih.gov/pubmed/20978109 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2350710 |
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