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In vivo protein stabilization based on fragment complementation and a split GFP system
Protein stabilization was achieved through in vivo screening based on the thermodynamic linkage between protein folding and fragment complementation. The split GFP system was found suitable to derive protein variants with enhanced stability due to the correlation between effects of mutations on the...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2010
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2993370/ https://ncbi.nlm.nih.gov/pubmed/21041669 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1005689107 |
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