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Fluorescent Labeling of Antibody Fragments Using Split GFP

Antibody fragments are easily isolated from in vitro selection systems, such as phage and yeast display. Lacking the Fc portion of the antibody, they are usually labeled using small peptide tags recognized by antibodies. In this paper we present an efficient method to fluorescently label single chai...

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Detalhes bibliográficos
Main Authors: Ferrara, Fortunato, Listwan, Pawel, Waldo, Geoffrey S., Bradbury, Andrew R. M.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3187779/
https://ncbi.nlm.nih.gov/pubmed/21998685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0025727
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