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Fluorescent Labeling of Antibody Fragments Using Split GFP

Antibody fragments are easily isolated from in vitro selection systems, such as phage and yeast display. Lacking the Fc portion of the antibody, they are usually labeled using small peptide tags recognized by antibodies. In this paper we present an efficient method to fluorescently label single chai...

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Main Authors: Ferrara, Fortunato, Listwan, Pawel, Waldo, Geoffrey S., Bradbury, Andrew R. M.
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2011
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3187779/
https://ncbi.nlm.nih.gov/pubmed/21998685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0025727
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