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Fluorescent Labeling of Antibody Fragments Using Split GFP

Antibody fragments are easily isolated from in vitro selection systems, such as phage and yeast display. Lacking the Fc portion of the antibody, they are usually labeled using small peptide tags recognized by antibodies. In this paper we present an efficient method to fluorescently label single chai...

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Bibliographische Detailangaben
Hauptverfasser: Ferrara, Fortunato, Listwan, Pawel, Waldo, Geoffrey S., Bradbury, Andrew R. M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3187779/
https://ncbi.nlm.nih.gov/pubmed/21998685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0025727
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