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In vivo protein stabilization based on fragment complementation and a split GFP system

Protein stabilization was achieved through in vivo screening based on the thermodynamic linkage between protein folding and fragment complementation. The split GFP system was found suitable to derive protein variants with enhanced stability due to the correlation between effects of mutations on the...

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Detalles Bibliográficos
Main Authors: Lindman, Stina, Hernandez-Garcia, Armando, Szczepankiewicz, Olga, Frohm, Birgitta, Linse, Sara
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2993370/
https://ncbi.nlm.nih.gov/pubmed/21041669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1005689107
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