Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.

A DNA-transfection protocol has been developed that makes use of a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA). Small unilamellar liposomes containing DOTMA interact spontaneously with DNA to form lipid-DNA complexes with 100% entrapment of the DNA...

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Библиографические подробности
Опубликовано в: :Proc Natl Acad Sci U S A
Главные авторы: Felgner, P L, Gadek, T R, Holm, M, Roman, R, Chan, H W, Wenz, M, Northrop, J P, Ringold, G M, Danielsen, M
Формат: Artigo
Язык:Inglês
Опубликовано: National Academy of Sciences 1987
Предметы:
Research Article
Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299306/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2823261/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.21.7413
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