Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure.
Efficient transfection of eukaryotic cells is dependent on the purity of the transfected genomic DNA. In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate. The DNA obtaine...
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| 發表在: | Nucleic Acids Res |
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| Principais autores: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
1990
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| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330274/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2326165/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.2.351 |
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