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Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure.

Efficient transfection of eukaryotic cells is dependent on the purity of the transfected genomic DNA. In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate. The DNA obtaine...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Main Authors: Alberti, S, Fornaro, M
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1990
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330274/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2326165/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.2.351
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