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Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure.
Efficient transfection of eukaryotic cells is dependent on the purity of the transfected genomic DNA. In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate. The DNA obtaine...
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| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
1990
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| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC330274/ https://ncbi.nlm.nih.gov/pubmed/2326165 |
| Etiquetas: |
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