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Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure.

Efficient transfection of eukaryotic cells is dependent on the purity of the transfected genomic DNA. In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate. The DNA obtaine...

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Sparad:
Bibliografiska uppgifter
Huvudupphovsmän: Alberti, S, Fornaro, M
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1990
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC330274/
https://ncbi.nlm.nih.gov/pubmed/2326165
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