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Accurate modification of a chromosomal plasmid by homologous recombination in human cells.
We have examined the consequences of modifying mammalian cellular DNA sequences by homologous recombination. A plasmid carrying a 248-base-pair deletion in the neomycin phosphotransferase (neo) gene was introduced into hamster and human cells. The integrated, defective neo gene was used as a target...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1987
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299176/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2821545/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.19.6820 |
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