Cancer Proliferation Gene Discovery Through Functional Genomics

Retroviral short hairpin RNA (shRNA)–mediated genetic screens in mammalian cells are powerful tools for discovering loss-of-function phenotypes. We describe a highly parallel multiplex methodology for screening large pools of shRNAs using half-hairpin barcodes for microarray deconvolution. We carrie...

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Detalhes bibliográficos
Main Authors: Schlabach, Michael R., Luo, Ji, Solimini, Nicole L., Hu, Guang, Xu, Qikai, Li, Mamie Z., Zhao, Zhenming, Smogorzewska, Agata, Sowa, Mathew E., Ang, Xiaolu L., Westbrook, Thomas F., Liang, Anthony C., Chang, Kenneth, Hackett, Jennifer A., Harper, J. Wade, Hannon, Gregory J., Elledge, Stephen J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2981870/
https://ncbi.nlm.nih.gov/pubmed/18239126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1149200
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