Stabilization of phage T4 lysozyme by engineered disulfide bonds.
Four different disulfide bridges (linking positions 9-164, 21-142, 90-122, and 127-154) were introduced into a cysteine-free phage T4 lysozyme at sites suggested by theoretical calculations and computer modeling. The new cysteines spontaneously formed disulfide bonds on exposure to air in vitro. In...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1989
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297884/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2671995/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.17.6562 |
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