Stabilization of phage T4 lysozyme by engineered disulfide bonds.

Four different disulfide bridges (linking positions 9-164, 21-142, 90-122, and 127-154) were introduced into a cysteine-free phage T4 lysozyme at sites suggested by theoretical calculations and computer modeling. The new cysteines spontaneously formed disulfide bonds on exposure to air in vitro. In...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Matsumura, M, Becktel, W J, Levitt, M, Matthews, B W
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1989
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297884/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2671995/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.17.6562
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