Probing the oxyferrous and catalytically active ferryl states of Amphitrite ornata dehaloperoxidase by cryoreduction and EPR/ENDOR spectroscopy. Detection of Compound I

Dehaloperoxidase (DHP) from Amphitrite ornata is a heme protein that can function both as a hemoglobin and a peroxidase. This report describes the use of 77 K cryoreduction EPR/ENDOR techniques to study both functions of DHP. Cryoreduced oxyferrous [Fe(II)-O(2)] DHP exhibits two EPR signals characte...

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Bibliografiske detaljer
Main Authors: Davydov, Roman, Osborne, Robert L., Shanmugam, Muralidharan, Du, Jing, Dawson, John H., Hoffman, Brian M.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2978736/
https://ncbi.nlm.nih.gov/pubmed/20925340
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja1059747
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