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Production of simian virus 40 large tumor antigen in bacteria: altered DNA-binding specificity and dna-replication activity of underphosphorylated large tumor antigen.

A bacterial expression system was used to produce simian virus 40 large tumor antigen (T antigen) in the absence of the extensive posttranslational modifications that occur in mammalian cells. Wild-type T antigen produced in bacteria retained a specific subset of the biochemical activities displayed...

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Uloženo v:
Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Mohr, I J, Gluzman, Y, Fairman, M P, Strauss, M, McVey, D, Stillman, B, Gerard, R D
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 1989
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297867/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2549538/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.17.6479
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