Production of simian virus 40 large tumor antigen in bacteria: altered DNA-binding specificity and dna-replication activity of underphosphorylated large tumor antigen.
A bacterial expression system was used to produce simian virus 40 large tumor antigen (T antigen) in the absence of the extensive posttranslational modifications that occur in mammalian cells. Wild-type T antigen produced in bacteria retained a specific subset of the biochemical activities displayed...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1989
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297867/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2549538/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.17.6479 |
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