Quantitative Assessment of the Sensitivity of Various Commercial Reverse Transcriptases Based on Armored HIV RNA

BACKGROUND: The in-vitro reverse transcription of RNA to its complementary DNA, catalyzed by the enzyme reverse transcriptase, is the most fundamental step in the quantitative RNA detection in genomic studies. As such, this step should be as analytically sensitive, efficient and reproducible as poss...

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Detalhes bibliográficos
Main Authors: Okello, John B. A., Rodriguez, Linda, Poinar, Debi, Bos, Kirsten, Okwi, Andrew L., Bimenya, Gabriel S., Sewankambo, Nelson K., Henry, Kenneth R., Kuch, Melanie, Poinar, Hendrik N.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2010
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2978101/
https://ncbi.nlm.nih.gov/pubmed/21085668
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0013931
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