Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR

BACKGROUND: In functional genomics, transcript measurement is of fundamental importance. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays are the most popular technology and depend on the initial molecular step, the reverse transcription (RT). This study provides a compl...

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Bibliographic Details
Main Authors: Levesque-Sergerie, Jean-Philippe, Duquette, Mathieu, Thibault, Catherine, Delbecchi, Louis, Bissonnette, Nathalie
Format: Artigo
Language:Inglês
Published: BioMed Central 2007
Subjects:
Methodology Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2151766/
https://ncbi.nlm.nih.gov/pubmed/17953766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-8-93
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