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Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
BACKGROUND: Reverse transcriptase is an indispensable enzyme for real-time reverse transcriptase (RT)-PCR, a standard method in molecular diagnostics for detection and quantification of defined RNA molecules. The prevention of non-specific products due to elongation of misprimed oligonucleotides by...
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| Publicado no: | J Anal Sci Technol |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Korea Basic Science Institute
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7099353/ https://ncbi.nlm.nih.gov/pubmed/32226638 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40543-015-0063-4 |
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