Cost-Effective Real-Time Reverse Transcriptase PCR (RT-PCR) To Screen for Dengue Virus followed by Rapid Single-Tube Multiplex RT-PCR for Serotyping of the Virus

Virus detection methodology provides detection of dengue virus in the early phase of the disease. PCR, targeting cDNA derived from viral RNA, has been used as a laboratory-based molecular tool for the detection of Dengue virus. We report the development and use of three real-time one-step reverse tr...

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Bibliographic Details
Main Authors: Lai, Yee-Ling, Chung, Youne-Kow, Tan, Hwee-Cheng, Yap, Hoon-Fang, Yap, Grace, Ooi, Eng-Eong, Ng, Lee-Ching
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2007
Subjects:
Virology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1829098/
https://ncbi.nlm.nih.gov/pubmed/17215345
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01258-06
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