Efficient construction of long DNA duplexes with internal non-Watson–Crick motifs and modifications
We have developed a semi-synthetic approach for preparing long stretches of DNA (>100 bp) containing internal chemical modifications and/or non-Watson–Crick structural motifs which relies on splint-free, cell-free DNA ligations and recycling of side-products by non-PCR thermal cycling. A double-stra...
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| Izdano u: | Nucleic Acids Res |
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| Glavni autori: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
2001
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC29687/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11139636/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.2.e6 |
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