Efficient construction of long DNA duplexes with internal non-Watson–Crick motifs and modifications
We have developed a semi-synthetic approach for preparing long stretches of DNA (>100 bp) containing internal chemical modifications and/or non-Watson–Crick structural motifs which relies on splint-free, cell-free DNA ligations and recycling of side-products by non-PCR thermal cycling. A double-stra...
Na minha lista:
| Publicado no: | Nucleic Acids Res |
|---|---|
| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2001
|
| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC29687/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11139636/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.2.e6 |
| Tags: |
Sem tags, seja o primeiro a adicionar uma tag!
|