Efficient construction of long DNA duplexes with internal non-Watson–Crick motifs and modifications

We have developed a semi-synthetic approach for preparing long stretches of DNA (>100 bp) containing internal chemical modifications and/or non-Watson–Crick structural motifs which relies on splint-free, cell-free DNA ligations and recycling of side-products by non-PCR thermal cycling. A double-stra...

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Bibliografische gegevens

Gepubliceerd in:	Nucleic Acids Res
Hoofdauteurs:	Zheng, Xiaofeng, Bevilacqua, Philip C.
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	Oxford University Press 2001
Onderwerpen:	NAR Methods Online
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC29687/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11139636/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.2.e6
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