Efficient construction of long DNA duplexes with internal non-Watson–Crick motifs and modifications
We have developed a semi-synthetic approach for preparing long stretches of DNA (>100 bp) containing internal chemical modifications and/or non-Watson–Crick structural motifs which relies on splint-free, cell-free DNA ligations and recycling of side-products by non-PCR thermal cycling. A double-stra...
Kaydedildi:
| Yayımlandı: | Nucleic Acids Res |
|---|---|
| Asıl Yazarlar: | , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
2001
|
| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC29687/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11139636/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.2.e6 |
| Etiketler: |
Etiket eklenmemiş, İlk siz ekleyin!
|