Effect of Long-Term Storage in TRIzol on Microarray-Based Gene Expression Profiling

BACKGROUND: Although TRIzol is widely used for preservation and isolation of RNA, there is suspicion that prolonged sample storage in TRIzol may affect array-based gene expression profiling (GEP), via premature termination during reverse transcription (RT). METHODS: GEP on Illumina arrays compared p...

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Hauptverfasser: Ma, Wencai, Wang, Michael, Wang, Zhi-Qiang, Sun, Luhong, Graber, David, Matthews, Jairo, Champlin, Richard, Yi, Qing, Orlowski, Robert Z., Kwak, Larry W., Weber, Donna M., Thomas, Sheeba K., Shah, Jatin, Kornblau, Steven, Davis, R. Eric
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2966770/
https://ncbi.nlm.nih.gov/pubmed/20805315
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1158/1055-9965.EPI-10-0565
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