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Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes

BACKGROUND: RNA interference (RNAi) is a powerful approach to study a gene function. Transgenic RNAi is an adaptation of this approach where suppression of a specific gene is achieved by expression of an RNA hairpin from a transgene. In somatic cells, where a long double-stranded RNA (dsRNA) longer...

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Detalhes bibliográficos
Main Authors: Sarnova, Lenka, Malik, Radek, Sedlacek, Radislav, Svoboda, Petr
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2964603/
https://ncbi.nlm.nih.gov/pubmed/20939886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-5751-9-8
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