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Reversed DNA Strand Cleavage Specificity in Initiation of Cre–LoxP Recombination Induced by the His289Ala Active-site Substitution

During the first steps of site-specific recombination, Cre protein cleaves and religates a specific homologous pair of LoxP strands to form a Holliday junction (HJ) intermediate. The HJ is resolved into recombination products through exchange of the second homologous strand pair. CreH289A, containin...

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Autores principales: Gelato, Kathy A., Martin, Shelley S., Baldwin, Enoch P.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2005
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2964137/
https://ncbi.nlm.nih.gov/pubmed/16242714
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2005.08.077
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