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A Kinetic Assay of Mitochondrial ATP-ADP Exchange Rate in Permeabilized Cells
We have previously described a method to measure ADP-ATP exchange rates in isolated mitochondria by recording the changes in free extramitochondrial [Mg(2+)] reported by a Mg(2+)-sensitive fluorescent indicator, exploiting the differential affinity of ADP and ATP to Mg(2+). In this manuscript we des...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2010
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2943973/ https://ncbi.nlm.nih.gov/pubmed/20691655 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.07.031 |
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