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A Kinetic Assay of Mitochondrial ATP-ADP Exchange Rate in Permeabilized Cells

We have previously described a method to measure ADP-ATP exchange rates in isolated mitochondria by recording the changes in free extramitochondrial [Mg(2+)] reported by a Mg(2+)-sensitive fluorescent indicator, exploiting the differential affinity of ADP and ATP to Mg(2+). In this manuscript we des...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Kawamata, Hibiki, Starkov, Anatoly A, Manfredi, Giovanni, Chinopoulos, Christos
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2943973/
https://ncbi.nlm.nih.gov/pubmed/20691655
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.07.031
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